Journal of Mechanical Engineering >

2019 , Vol. 55 >Issue 2: 1 - 9

DOI: https://doi.org/10.3901/JME.2019.02.001

Electrical Impedance Tomography for Biological Cell Sensing with Microfluidic Device

  • YAO Jiafeng ,
  • LIU Xiayi ,
  • XU Zifei ,
  • ZHAO Tong ,
  • CHEN Bai ,
  • WU Hongtao
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  • 1. College of Mechanical & Electrical Engineering, Nanjing University of Aeronautics & Astronautics, Nanjing 210016;
    2. Faculty of Mechanical and Precision Instrument Engineering, Xi'an University of Technology, Xi'an 710048;
    3. Department of Mechanical Engineering, Chiba University, Chiba 263-0022, Japan

Received date: 2017-09-13

  Revised date: 2018-08-24

  Online published: 2019-01-20

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Abstract

A micro electrical impedance tomography (μEIT) system is developed to visualize cells concentration distribution in microchannel flow. Due to the complexity of electrical properties of the μEIT system in micro-scale measurement, simulation and experiments are conducted to find the optimal conditions of the image reconstruction process. In the simulation, three image reconstruction algorithms which are generalized vector sampled pattern matching (GVSPM), iterative tikhonov regularization (TK) and projected landweber iteration (PLW) are estimated, GVSPM is found to be the optimal algorithm for image reconstruction in the present study due to its higher image correlation IC=0.84 and lower image error IE=0.43. In the experiment, yeast cells and purified water are employed as two-phase flow to measure the cells sedimentation in the microchannel at a range of frequencies with GVSPM, TK and PLW, respectively. The optimal frequency for the μEIT system is found as f=1 MHz due to its higher measurement sensitivity. GVSPM is found as the optimal image reconstruction algorithm because of its low voltage error UE=0.582 and simpler image reconstruction without regularization factor. Finally, images of cells sedimentation are reconstructed with GVSPM in three cross-sections in microchannel flow at f=1 MHz. The reconstructed images show that concentration of cells sedimentation from the upstream Z1 to downstream z5 is decreased gradually along the flow direction in the microchannel.

Key words： image reconstruction algorithm; potential drop; cell electrical properties; cell sedimentation; two-phase flow

Cite this article

YAO Jiafeng , LIU Xiayi , XU Zifei , ZHAO Tong , CHEN Bai , WU Hongtao . Electrical Impedance Tomography for Biological Cell Sensing with Microfluidic Device[J]. Journal of Mechanical Engineering, 2019 , 55(2) : 1 -9 . DOI: 10.3901/JME.2019.02.001

References

[1] YANG Y,JIA J,SMITH S,et al. A miniature electrical impedance tomography sensor and 3d image reconstruction for Cell Imaging[J]. IEEE Sensors Journal,2016(99):1.
[2] PRETLOW T G,PRETLOW T P. Velocity sedimentation of cells[J]. Nature,1988,333(6168):97-97.
[3] 闫姿姿,李姗姗,李铭浩,等. 交流电热流场对微纳粒子介电泳行为的影响[J]. 机械工程学报,2017,53(14):202-208. YAN Zizi,LI Shanshan,LI Minghao,et al. Influence of alternating current electrothermal flow field on the behavior of micro nano particle dielectrophoresis[J]. Journal of Mechanical Engineering,2017,53(14):202-208.
[4] YU Kun,JIANG,HONG Yuan. Continuous separation of multiple size microparticles using alternating current dielectrophoresis in microfluidic device with acupuncture needle electrodes[J]. Chinese Journal of Mechanical Engineering,2016,29(2):325-331.
[5] LECRIVAIN G,BARRY L,HAMPEL U. Three-dimensional simulation of multilayer particle deposition in an obstructed channel flow[J]. Powder Technology,2014,258(6):134-143.
[6] YANG W. Design of electrical capacitance tomography sensors[J]. Measurement Science & Technology,2010,21(4):447-453.
[7] LEE E,TS M E,SEO J K,et al. Breast EIT using a new projected image reconstruction method with multi-frequency measurements[J]. Physiological Measurement,2012,33(5):751-765.
[8] ADLER A,AMATO M B,ARNOLD J H,et al. Whither lung EIT:where are we,where do we want to go and what do we need to get there?[J]. Physiological Measurement,2012,33(5):679-694.
[9] PARKS J W,OLSON M A,KIM J,et al. Integration of programmable microfluidics and on-chip fluorescence detection for biosensing applications[J]. Biomicrofluidics,2014,8(5):151-184.
[10] ASAMI K. Low-frequency dielectric dispersion of bacterial cell suspensions[J]. Colloids & Surfaces B Biointerfaces,2014,119(119):1-5.
[11] HUA X,MARSHALL M J,XIONG Y,et al. Two-dimensional and three-dimensional dynamic imaging of live biofilms in a microchannel by time-of-flight secondary ion mass spectrometry[J]. Biomicrofluidics,2015,9(3):623-633.
[12] YORK T A,PHUA T N,REICHELT L,et al. A miniature electrical capacitance tomograph[J]. Measurement Science & Technology,2006,17(8):2119-2129.
[13] SUN T,TSUDA S,ZAUNER K-P,et al. On-chip electrical impedance tomography for imaging biological cells[J]. Biosens Bioelectron,2010,25(5):1109-1115.
[14] MEIR A,RUBINSKY B. Electrical impedance tomographic imaging of a single cell electroporation[J]. Biomed Microdevices,2014,16(3):427-437.
[15] CHAI K T C,DAVIES J H,CUMMING D R S. Electrical impedance tomography for sensing with integrated microelectrodes on a CMOS microchip[J]. Sens Actuators,B,2007,127(1):97-101.
[16] ASAMI K,YONEZAWA T,WAKAMATSU H,et al. Dielectric spectroscopy of biological cells[J]. Bioelectrochem Bioenerg,1996,40(2):141-145.
[17] XIAO C,LACHANCE B,SUNAHARA G,et al. An in-depth analysis of electric cell-substrate impedance sensing to study the attachment and spreading of mammalian cells[J]. Analytical Chemistry,2002,74(6):1333-1339.
[18] YANG W. Hardware design of electrical capacitance tomography systems[J]. Measurement Science & Technology,1996,7(3):225-232.
[19] ANDíA B I,SAUER K D,BOUMAN C A. Nonlinear backprojection for tomographic reconstruction[J]. IEEE Transaction on Nuclear Science,2002,49(1):61-68.
[20] YANG W,PENG L. Image reconstruction algorithms for electrical capacitance tomography[J]. Journal of Tsinghua University,2007,14(4):478-484.
[21] 许军,李坤. 电接触的接触电阻研究[J]. 电工材料,2011(1):10-13. XU Jun,LI Kun. Study on contact resistance of electrical contact[J]. Electrical Engineering Materials,2011(1):10-13.
[22] CASELLI F,BISEGNA P,MACERI F. EIT-inspired microfluidic cytometer for single-cell dielectric spectroscopy[J]. Journal of Microelectromechanical Systems,2010,19(5):1029-1040.
[23] YAO J,OBARA H,SAPKOTA A,et al. Development of three-dimensional integrated microchannel-electrode system to understand the particles' movement with electrokinetics[J]. Biomicrofluidics,2016,10(2):87-105.
[24] YAO J,KODERA T,OBARA H,et al. Spatial concentration distribution analysis of cells in electrode-multilayered microchannel by dielectric property measurement[J]. Biomicrofluidics,2015,36(4):014125.
[25] TAKEI M. GVSPM image reconstruction for capacitance CT images of particles in a vertical pipe and comparison with the conventional method[J]. Measurement Science & Technology,2006,17(8):2104-2112.
[26] PENG L,YE J,LU G,et al. Evaluation of effect of number of electrodes in ECT sensors on image quality[J]. IEEE Sensors Journal,2012,12(5):1554-1565.
[27] 王化祥,王超,陈磊. 基于Landweber迭代的图像重建算法[J]. 信号处理,2000,16(4):354-356. WANG Huaxiang,WANG Chao,CHEN Lei. Image reconstruction algorithm based on Landweber iteration[J]. Signal Processing,2000,16(4):354-356.
[28] ZHANG H,CHENG L. Projected Landweber iteration for matrix completion[J]. Journal of Computational & Applied Mathematics,2010,235(3):593-601.
[29] YAO J,SAPKOTA A,KONNO H,et al. Noninvasive online measurement of particle size and concentration in liquid-particle mixture by estimating equivalent circuit of electrical double layer[J]. Partticulate Science & Technology,2015,34(5):517-525.
[30] 谭德坤,刘莹. 双电层效应对压力驱动微流体流动及传热的影响[J]. 机械工程学报,2012,48(18):144-151. TAN Dekun,LIU Ying. Effect of electric double layer effect on pressure driven microfluidic flow and heat transfer[J]. Journal of Mechanical Engineering,2012,48(18):144-151.
[31] SANT S. Physics and chemistry of interfaces[J]. Advanced Manufacturing Processes,2003,28(12):1379-1380.
[32] ASAMI K,SEKINE K. Dielectric modelling of cell division for budding and fission yeast[J]. Journal of Physics D Applied Physics,2007,40(4):1128-1133.
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