Enhancing the groove drainage ability is one of the main ways to improve the tire anti-hydroplaning performance. Inspired by the bionic theory, the pit bionic non-smooth structure is introduced into tire groove and bionic method for enhance groove drainage ability by reducing fluid resistance is explored. The single tire groove of contact area is extracted for analysis and the pit bionic non-smooth structure is arranged on the bottom of tire groove. The computational fluid dynamics(CFD) and orthogonal experimental design are used to study the influence of pit bionic non-smooth structure on wall drag reduction, and the optimal parameters combination is determined. To further enhance the drag reduction rate of pit bionic non-smooth structure, a water drop shaped pit boinic structure is proposed and its drag reduction characteristic is analyzed. The results show the drag reduction effect of the water drop shaped pit boinic structure is better than spherical pit structure. The optimal water drop shaped pit structure is arranged on the bottom of tire groove for anti-hydroplaning analysis. The results show that the water drop shaped pit boinic structure can reduce flow resistance and improve the groove drainage volume. Therefore, it can decrease the tread hydrodynamic pressure when driving on water film and raise the tire anti-hydroplaning performance.