LIN Chengxu, GAN Lang, TAN Xinping, LI Xuemei, YAN Wang, LI Qian, CHEN Gang, LIAO Guanglan, LIU Zhiyong
FISH technology was a common method to detect the nucleic acid sequence of malignant tumor cells. It was widely used in the field of cancer diagnoses and treatments. In order to realize the full automation of FISH experiments, a full-automatic pathological staining system was designed, which was provided with multi axis control arm, reagent sampler, slide, cover slide fixture, auxiliary module and so on. The positioning erorrs of the multi axis control arm are less than ±0.1 mm, the accuracy of extracting and discarding the pipette head is more than 99.5%, the success rate of extracting the cover glass is more than 99%, and the erorrs of micro reagent sampling are less than 0.6 μL. The errors of adding a large amount of reagent are less than 0.5 mL, the temperature control erorrs of slide clamp are less than ±1 ℃, and there is no reagent leakage. The results of biological experiments show that the system has good fluorescence staining effectiveness, the signal points are clearly visible, and the interpretable rate of fluorescence image is more than 90%.