MoS2/Ni60A solid self-lubricating coating was prepared on 35CrMnSi steel substrate by laser cladding with Ni60A alloy powder and MoS2 powder as cladding materials. The effects of MoS2 addition amount (mass fraction of 1%, 3%, 5%, 7%, 9%) on the phase composition, microstructure, microhardness and tribological properties of the coating were investigated. The results show that the coatings with different MoS2 addition had good surface formability and were metallurgical by bonded with the substrate. The main phase of the coatings were MoS2, γ-Ni, NiS, Cr2B. The microstructure of middle and upper position was mainly composed of rod-shaped, fishbone-shaped and radial dendrite. The microstructure of the bottom and fusion line was composed of planar and columnar crystals. With the increase of MoS2 addition, the microhardness of the coating decreased and the wear rate increased, and the friction coefficient decreased first and then increased. When the addition of MoS2 was 3%, the friction coefficient of the coating reached the lowest of 0.43, and a complete solid self-lubricating film was formed on the worn surface; The plough furrow was less and the main wear mechanism was slight abrasive wear.