The resistance spot welding was conducted on 1.5 mm thick GH163 nickel-based superalloy, and the effects of welding current, electrode pressure and welding time on the microstructure, microhardness and tensile shear of the joint were studied. The relatively good welding parameters were obtained. The results show that the fine dendritic columnar crystals was uniformly distributed in the nugget zone of the joint, with the growth direction perpendicular to the nugget arc line, which was the same direction as the heat radiating of the nugget. Meanwhile with the current increase, the grain size decreased. The microhardness of the joint increased with the shortening distance to the nugget center while showed an increasing trend with the increasing welding current. The tensile shear of the joint first increased then decreased, and then increased again with the welding current increase, and gradually increased with the prolongation of welding time while decreased with the increase of electrode pressure. When the electrode pressure was 11.0 kN, welding current was 7.1 kA and welding time was 0.50 s, the comprehensive properties of the joint were the best, with the nugget diameter of 7.14 mm, the room and high temperature tensile shear force of 15.34 kN and 13.13 kN, and the tensile fracture mode of base metal fracture.